Agarose multipurpose, white powder for molecular biology
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
Agarose is a natural polysaccharide isolated from agar or agar-bearing marine algae. Its physical and chemical properties are particularly suited for use as a gel medium for diffusion and electrokinetic movement of biomolecules. Agarose is essentially biologically inert and forms relatively clear, transparent gels. Agarose based gels offer several advantages: High gel strength permits use of low concentrations. The macroporous nature of the gels allows rapid diffusion of high molecular weight molecules. Agarose is Non-toxic. Agarose gels are thermally reversible facilitating sample recovery. Staining and destaining may be done rapidly with minimal background. Because agarose forms macroporous gels that are electrically nonionic, it is an excellent medium for Isoelectric Focusing (IEF). A selection of different formulated agaroses with specific qualities for unique applications are available from MP.
Multipurpose Agarose is a versatile high gel strength agarose, especially designed for a wide range of molecular biology techniques. It is used for resolution of fragments over a large size range from 200 bp up to 50 kb by conventional electrophoresis. It is suitable for use in pulsed field gel electrophoresis and for blotting assays.
High gel strength agarose, especially designed for a wide range DNA. Versatile – separate fragments from 200 bp up to 50 kb by conventional electrophoresis. Concentrations between 0.4% and 6%. Multipurpose Agarose features the highest gel strength available, allowing 0.4 - 0.5% agarose gels. Significantly decreases electrophoresis times. Improves separation of very large fragments. Dissolves easily in a microwave.
- Molecular Biology Grade
- Presentation: White Powder
- pH in Solution: 8.21 pH in Gel: 7.98
- Soluble in water (1.5 g plus 100 mL)
|
Melting Pt:
89 °C ± 1.5 °C (1.5% gel) Storage Temperature: Ambient |
MDL Number:
MFCD00081294 CAS Number: 9012-36-6 |
|
Specification Test Results
| Identity Test | Passes |
| Gelling Temperature (1.5% gel) | 36 ± 1.5°C |
| Melting Temperature (1.5% gel) | 89 ± 1.5°C |
| Gel Strength (1.5% gel) | > 3200 g/cm2 |
| Electroendosmosis | <0.12% |
| Sulfate | <0.07% |
| DNase/Rnase | None Detected |
| DNA Blotting | Complete Transfer |
| Inhibition of Ligation and Restriction Enzymes | None Detected |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...