X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) ≥98%, white powder for molecular biology
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
Synonyms:
BCIG
, X-GAL
, (2S,3R,4S,5R,6R)-2-((5-Bromo-4-chloro-1H-indol-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.
- Molecular Biology Reagent Grade
- Optical Rotation: (c=1, DMF:H2O 1:1): -62.0 ° (Lit.)
- Soluble in 50 mg/mL, DMF : MeOH (1:1). Soluble in DMF (2% w/v - clear, colorless solution).
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Formula:
C₁₄H₁₅BrClNO₆ MW: 408.63 g/mol Melting Pt: 230 °C (dec., lit.) Storage Temperature: Freezer |
MDL Number:
MFCD00005666 CAS Number: 7240-90-6 |
Specification Test Results
| Identity Test | Passes |
| Purity | ≥98% |
| FTIR | Conforms to Standard |
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