Quickly purify high yields of high-molecular-weight genomic DNA, total cellular RNA or Total Nucleic Acid (TNA) with one kit
The MasterPure™ Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types.
The kit procedure uses a safe, gentle, and scalable salt-precipitation protocol that eliminates the need for hazardous chemicals and yield-reducing columns. Purification reactions can be scaled up or scaled down depending on the amount of nucleic acid needed and the size of the sample.
Applications include:
Library preparation for next generation sequencing (NGS) of genomic DNA and RNA
DNA methylation studies using Illumina® Infinium® HumanMethylation BeadChips
Genomic DNA and cDNA cloning
qPCR and qRT-PCR
Micrarray analyses (CGH, gene expression profiling, etc)
Fast:Purify Total Nucleic Acid (TNA), DNA or RNA in 30 – 60 minutes
Versatile: Purify TNA, genomic DNA, total RNA, FFPE RNA, or both genomic DNA and total RNA from a sample
Safe: Does not use hazardous phenol, chloroform or guanidine
High Purity: A260/A280 ratios consistently between 1.8 and 2.0
High Yields: Improves yields by avoiding the use of columns which often reduce nucleic acid yields
Total RNA Recovery: Purify both large and small (e.g., miRNA) RNA for RNA-Seq or qRT-PCR
Proven: Hundreds of citations for purification of DNA and RNA from dozens of sample types for use in many applications