Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.
Western blot: 1:15000; In-Cell Western Assay: 1:800
Type: Secondary
Antigen: IgG
Clonality: Polyclonal
Clone:
Conjugation: IRDye 680RD
Epitope:
Host: Goat
Isotype:
Reactivity: Rabbit