Dependable, consistent high-fidelity PCR results with every target DNA template, every time
- A complete, ready-to-use kit
- PCR amplification of difficult or high-GC templates
- Multiplex PCR compatible
- Robust amplification of targets up to 20 kb long
- High accuracy with 3-fold lower error rate than Taq DNA polymerase (error rate = 1 in 30,000)
- Extremely high sensitivity and specificity using the PCR Enhancer Technology (with Betaine)
The FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence
How the FailSafe System Works
1. First, purchase the FailSafe PCR PreMix Selection Kit (catalog number FS99060). The FailSafe PCR PreMix Selection Kit is composed of:
FailSafe PCR Enzyme Blend (60 Units)
Twelve (12) different FailSafe™ 2X PCR PreMixes. Each 2X PreMix contains dNTPs, Buffer and different concentrations of Mg+2 and Epicentre's proprietary PCR Enhancer (with Betaine).
2. Prepare and perform PCR reactions using your template and PCR primers with each of the 12 FailSafe™ 2X PCR PreMixes and the FailSafe PCR Enzyme.
3. Analyze, by agarose gel electrophoresis, the PCR product produced from each of the 12 reactions and visually determine which PreMix gives the best amplification (e.g., a single, strong PCR product of the desired length or "as expected" multiplex PCR results). We guarantee that one of the FailSafe PCR 2X PreMixes will give the desired amplification product.
4. For reliable and consistent PCR results, use the FailSafe PCR Enzyme and the best 2X PreMix indentified in the optimization experiments for all subsequent amplifications with that template/primer combination.
5. To reduce costs, purchase a FailSafe PCR System (Cat. No. FS99100, FS99250,or FS9901K) which provides sufficient enzyme and the appropriate amount of your favorite 2X PCR Premix(es) instead of buying the enzyme and 2X PCR Premixes separately.
Certifications: For research use only. Not for human or diagnostic use.
Ordering information: The FailSafe™ PCR PreMix Selection Kit includes FailSafe™ PCR Enzyme Mix and all 12 FailSafe™ PCR 2X PreMixes. Each 2X Premix includes dNTPs, buffer, and various amounts of MgCl2 and FailSafe™ PCR Enhancer with Betaine
The FailSafe™ PCR System with PreMix Choice (any 1 PreMix) includes 100 units of enzyme and you choose/order any one of the FailSafe™ PCR 2X PreMixes from the list.
The FailSafe™ PCR System with PreMix Choice (any 2 PreMixes) includes 250 units of enzyme and you choose/order any two FailSafe™ PCR 2X PreMixes from the list.
The FailSafe™ PCR System with PreMix Choice (any 8 PreMixes) includes 1000 units of enzyme and you choose/order any eight FailSafe™ PCR 2X PreMixes from the list.
Caution: FailSafe™ PCR Premix Selection Kits: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights (such as 5´ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
