Completely digest single-stranded RNA with this heat labile, non-sequence specific RNase
- Complete: Unlike RNase A, RNase I does not have a base preference and digests between all dinucleotide pairs in single-stranded RNA
- Heat Labile: RNase I is easily inactivated by heating 70°C for 15 minutes
RNase I degrades single-stranded RNA to nucleoside 3´ monophosphates via 2´,3´ cyclic monophosphate intermediates by cleaving between all dinucleotide pairs, unlike RNase A, which cleaves only after cytosine and uridine
Applications include:
Removal of RNA from DNA preparations
RNase protection assays to detect single-basepair mismatches in RNA:RNA and RNA:DNA hybrids
In addition, the enzyme is completely inactivated by heating at 70°C for 15 minutes, eliminating the requirement to remove the enzyme prior to many subsequent procedures.
Certifications: For research use only. Not for human or diagnostic use.
Ordering information: Contents include dilution buffer, 10X TNE buffer, and 0.1 M DTT.