Specifically degrade the RNA in a DNA:RNA hybrid, without affecting DNA or unhybridized
- Optimal activity above 65°C and maintains activity as high as 95°C
- Highly specific for RNA in a RNA:DNA hybrid and will not digest free RNA or DNA
- Maximizes digestion sensitivity and selectivity while minimizing background due to nonspecific hybridization
Hybridase™ Thermostable RNase H specifically degrades the RNA in a DNA:RNA hybrid, without affecting DNA or unhybridized RNA
Applications include:
High-stringency hybrid selection
Diagnostic assays of specific target DNA sequences by isothermal probe amplification
Transcription-based amplification methods (e.g., NASBA® method)
High-stringency mapping of mRNA structure
Applications that require specific hydrolysis of the RNA in a DNA:RNA hybrid
In contrast to E. coli RNase H, which is rapidly inactivated at 55°C, Hybridase RNase H is active at high temperatures. It has optimal activity above 65°C and can be used at temperatures up to 95°C. The thermostability of the enzyme permits it to be used at temperatures that give the highest hybridization stringency for specific DNA:RNA heteroduplexes, maximizing sensitivity and selectivity while minimizing background due to nonspecific hybridization.
Certifications: For research use only. Not for human or diagnostic use.
