E. coli Exonuclease I

Supplier: Lucigen

T4 DNA polymerase, exonuclease minus kit

T4 DNA polymerase, exonuclease minus kit

X40520K

76081-722EA 656.1 USD

76081-722

E. coli Exonuclease I

Enzymes

Digest single-stranded DNA from the 3' to 5' end, but not double-stranded or dsDNA with short 3' overhangs

Specific: Processively digest ssDNA in a 3´ to 5´ direction without harming dsDNA - short 3´ overhangs in DNA are not digested
Flexible: Active under a wide variety of buffer conditions and can be added directly into most reaction mixes
Heat Inactivated: Destroy the enzyme activity by incubating at 80°C for 15 minutes

Exonuclease I digests ssDNA in a 3´ to 5´ direction,1-3 but does not digest dsDNA

Applications include:
Removal of residual ssDNA, including oligos, from reaction mixes
Removal of ssDNA from nucleic acid mixtures

Although it requires the presence of magnesium and a free 3´-hydroxyl terminus for activity, it is active under a wide variety of buffer conditions and can be added directly into most reaction mixes. Exonuclease I can be heat-inactivated by incubation at 80°C for 15 minutes.

Certifications: For research use only. Not for human or diagnostic use.

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