Polyadenylate 3' ends of RNA molecules for various downstream applications
- Quality Kit: High enzymatic purity, specificity, and activity
- Flexible: Use polyadenylated RNAs in a variety of applications such as stabilizing in vitro transcribed RNA and adding primer (oligo-dT) binding sites to any RNA for first-strand cDNA synthesis
- Fast and Easy: Simple protocol produces polyadenylated RNA molecules quickly
Poly(A) Polymerase uses ATP as a substrate for template-independent addition of adenosine monophosphate to the 3´-hydroxyl termini of RNA molecules. The Poly(A) Polymerase Tailing Kit provides the enzyme and other reagents for quickly and easily adding a poly(A) tail to the 3´ end of any RNA
Applications include:
Addition of a poly(A) tail to RNA synthesized in vitro in order to increase the stability of the RNA and enhance its ability to be translated in vivo after transfection or microinjection into eukaryotic cells
Addition of a poly(A) tail to an RNA molecule or a mixture of RNA molecules in order to provide a priming site for synthesis of first-strand cDNA using a primer with poly(dT) on its 3´ end
Synthesis of polyadenylated RNA for nucleic acid amplification methods or gene expression studies
3´-end labeling of RNA with radioactive A residues
Quantifying mRNA
Poly(A) Polymerase is encoded by an E. coli gene that has been cloned into a plasmid and overexpressed in an E. coli strain.
Certifications: For research use only. Not for human or diagnostic use.
Ordering information: Contents include Poly(A) Polymerase, 10X Reaction Buffer, 10 mM ATP, and Sterile RNase-Free Water.
