Pierce™ HisPur™ Ni-NTA Superflow Agarose, Thermo Scientific

Supplier: Thermo Fisher Scientific
HisPur™
25215 25217 25214 25215 25216 25217 25214 25216
PI25217EA 10578.62 USD
PI25217 PI25214 PI25216 PI25215 CAPI25214 CAPI25217 CAPI25216 CAPI25215
Pierce™ HisPur™ Ni-NTA Superflow Agarose, Thermo Scientific
Protein Purification Protein Purification Resins
Thermo Scientific Pierce HisPur™ Ni-NTA Superflow Agarose is a nitrilotriacetic acid modified Superflow 6 support charged with divalent nickel (Ni+2) used for FPLC purification of poly-histidine-tagged proteins.

  • Binds greater than 20 mg of His-tagged GFP per ml of resin at a linear flow rate of 300 cm/hour
  • Provides greater than 80% purity of eluted fractions when used to purify from lysates
  • Can be used to purify proteins under native and denaturing conditions
  • Highly crosslinked beads tolerate linear flow rates up to 1200 cm/hour
  • Maintains function in a wide variety of chemicals and pH conditions
  • Resin is stable through multiple cycles of cleaning and reuse

HisPur™ Ni-NTA Superflow Agarose is a durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications.
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