Produce fully RNase A-resistant RNA that is perfectly suited for SELEX binding assays and RNA aptamer identification, as well as antisense RNA and RNA interference (RNAi) experiments.
- Stable: DuraScript® RNA, produced with this kit, is completely resistant to RNase A degradation, thus protecting your valuable RNA
- Multi-application Compatible: Synthesize long or short RNase A resistant transcripts ready for aptamer screening using Selex or for RNA interference experiments
- High Yields: Produce at least 110 – 307 pmol of DuraScript® RNA depending on the size of the transcript
- Flexible: Accepts a variety of template DNAs with standard T7 promoters including linearized vectors, PCR products, cDNA, and dsDNA oligos
The DuraScribe® T7 Transcription Kit is an in vitro transcription kit that produces RNA - called DuraScript® RNA - that is completely resistant to RNase A digestion.
The DuraScribe T7 Transcription Kit includes DuraScribe T7 RNA Polymerase, an enhanced formulation of the Y639F mutant1 of T7 RNA Polymerase that efficiently incorporates 2´-Fluorine-CTP (2´-F-dCTP), 2´-Fluorine-UTP (2´-F-dUTP), ATP and GTP into RNase-resistant RNA transcripts called DuraScript RNA. The DuraScribe T7 RNA Polymerase uses the same T7 promoters as the wild-type T7 RNA Polymerase and is therefore compatible with previously constructed T7-driven transcription templates.
Delivery information: Each DuraScribe® T7 Transcription Kit contains: DuraScribe T7 Enzyme Mix, RNase-Free DuraScribe T7 10X Reaction Buffer, ATP, GTP, 2-F-dCTP, 2-F-dUTP, DNase I, DTT, Control Template DNA (linearized), Sterile Deionized Water.
Caution: For research use only. Not for human or diagnostic use.
