Generate the highest yields of RNA quickly and easily with these T7 RNA polymerase in vitro transcription kits.
- Fast: Produce maximal RNA yields in only 30 minutes
- High Yield: Produce ≤180 µg of unlabeled RNA or >130 µg of biotin-labeled RNA from only 1 µg of template DNA
- Flexible: Accepts a variety of template DNAs with T7 promoters including linearized vectors, PCR products, cDNA, and dsDNA oligos
- Scalable: Reactions can be scaled up to quickly produce milligram - gram amounts of RNA
- Multi-application compatible: Make long (≤9 kb) or short (<50 bases) RNA transcripts with the standard T7-Flash kits suitable for a variety of applications including: CRISPR (gRNA), in vitro translation, RNAi (shRNA, dsRNA), RNase protection, structural studies, and anti-sense experiments
The AmpliScribe™ T7-Flash™ Transcription Kits use a rapid, high-yield in vitro transcription technology
The AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit enables high-yield synthesis of biotin-RNA or biotin-antisense RNA (aRNA, also called cRNA) using the same technology as the standard AmpliScribe T7-Flash Kit. This kit incorporates Biotin-16-UTP directly into the RNA transcript, producing high yields of biotin-RNA from as little as 50 ng of input DNA template. These nonradioactive, biotin-labeled RNAs are suitable for a variety of applications including microarray (transcription profiling) studies, in situ hybridization, and blotting experiments.
An AmpliScribe T7-Flash Transcription reaction yields more RNA (180 µg per 20-µL reaction) in 30 minutes than other high-yield in vitro transcription kit reactions produce in 2 hours. These kits utilize proprietary enzyme formulations that enable the maximum possible yields of RNA from virtually any template that is downstream of the T7 RNA polymerase promoter, including sequences in linearized plasmids, double-stranded oligos or PCR products.
Delivery information: Each AmpliScribe™ T7-Flash™ Transcription Kit contains: AmpliScribe™ T7-Flash™ Enzyme Solution, AmpliScribe™ T7-Flash™ 10X Reaction Buffer, ATP, CTP, GTP and UTP Solutions, RNase-Free Water, RNase-Free DNase I, DTT, Control DNA Template (linearized), RiboGuard™ RNase Inhibitor.
Each AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit contains: AmpliScribe™ T7-Flash™ Enzyme Solution, RiboGuard™ RNase Inhibitor, AmpliScribe™ T7-Flash™ 10X Reaction Buffer, NTP/Biotin-UTP PreMix (ATP, CTP, GTP, UTP, and Biotin-16-UTP) Solution, RNase-Free Water, RNase-Free DNase I, DTT, Control DNA Template (linearized).
Caution: For research use only. Not for human or diagnostic use.
