Clone large fragments with T1-phage resistance.
- Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA
- Avoid T1 and T5 phage contamination
- Generate clones with inducible copy number using CopyControl™ vectors
- Achieve high colony counts with high efficiency electrocompetent cells (>1 x 1010 cfu/µg pUC19 DNA)
Phage T1-Resistant TransforMax™ EPI300™-T1<sup>R</sup> <italic>E
"Phage T1-Resistant TransforMax™ EPI300™-T1R E. coli have all the benefits of the highly versatile TransforMax EPI300™ E. coli competent cells with the addition of being resistant to bacteriophages T1 and T5 (tonA genotype). Once introduced into the lab environment, bacteriophage T1 rapidly lyses E. coli strains that are commonly used in cloning applications and results in significant lab downtime and the loss of valuable clones. Bacteriophage T1 is particularly difficult to eliminate from the lab and can lay dormant for many years. The tonA genotype protects the phage T1-resistant TransforMax EC100-T1R cells and valuable clones from attack by bacteriophage T1.
Like the standard TransforMax EPI300 E. coli, this strain has been specially engineered for use with Epicentre's CopyControl™ Cloning Systems. The cells contain an inducible mutant trfA gene whose gene product is required for initiation of replication from the oriV contained on the CopyControl pCC1™ Vectors or on clones that have been retrofitted with CopyControl capability using the EZ-Tn5™<oriV/KAN-2> Insertion Kit. This process allows TransforMax EPI300-T1R E. coli to maintain CopyControl vectors at single copy until induction occurs immediately before DNA purification. EPI300-T1R competent cells are recombination minus (recA) and endonuclease minus (endA), for high yield, high quality plasmid DNA preparations. The strain is also blue/white screening capable (lacZΔM15) and restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] for cloning methylated DNA.
Genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG trfA dhfr tonA
Transformation efficiency: >1 x 1010 cfu/µg pUC19 DNA.
coli</italic> have all the benefits of the highly versatile TransforMax EPI300™ <italic>E. coli</italic> competent cells with the addition of being resistant to bacteriophages T1 and T5 (tonA genotype). Once introduced into the lab environment, bacteriophage T1 rapidly lyses <italic>E. coli</italic> strains that are commonly used in cloning applications and results in significant lab downtime and the loss of valuable clones. Bacteriophage T1 is particularly difficult to eliminate from the lab and can lay dormant for many years. The tonA genotype protects the phage T1-resistant TransforMax EC100-T1<sup>R</sup> cells and valuable clones from attack by bacteriophage T1.~Like the standard TransforMax EPI300 <italic>E. coli</italic>, this strain has been specially engineered for use with Epicentre's CopyControl™ Cloning Systems. The cells contain an inducible mutant trfA gene whose gene product is required for initiation of replication from the oriV contained on the CopyControl pCC1™ Vectors or on clones that have been retrofitted with CopyControl capability using the EZ-Tn5™<lt/>oriV/KAN-2<gt/> Insertion Kit. This process allows TransforMax EPI300-T1<sup>R</sup> <italic>E. coli</italic> to maintain CopyControl vectors at single copy until induction occurs immediately before DNA purification. EPI300-T1<sup>R</sup> competent cells are recombination minus (recA) and endonuclease minus (endA), for high yield, high quality plasmid DNA preparations. The strain is also blue/white screening capable (lacZΔM15) and restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] for cloning methylated DNA.~Genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG trfA dhfr tonA~Transformation efficiency: <gt/>1 x 10<sup>10</sup> cfu/µg pUC19 DNA.
Delivery information: Each kit contains ten tubes with 100 µL of cells (enough cells for 20 transformations), CopyControl™ Induction Solution and pUC19 control DNA. The CopyControl™ Induction Solution is provided at a 1000X concentration and is filter sterilized.
Caution: For research use only. Not for human or diagnostic use.
