For quantitative determination of total antioxidant capacity (TAC) and evaluation of drug effects on TAC metabolism.
- Linear detection range from 1.5 to 1000 μM Trolox
- The procedure involves addition of a single working reagent and incubation for 10 minutes
- Can readily automated to process thousands of samples per day
An antioxidant is a molecule capable of slowing or preventing the oxidation of other molecules
BioAssay Systems’ improved assay measures total antioxidant capacity in which Cu2+ is reduced by antioxidant to Cu+. The resulting Cu+ specifically forms a colored complex with a dye reagent. The color intensity at 570nm is proportional to TAC in the sample.
Antioxidants protect the cells from damages by reactive oxygen species which are produced in oxidation reactions in the cell. Antioxidants can be small molecules such as glutathione, vitamins, or macromolecules such as catalase, glutathione peroxidase. As oxidative stress contributes to the development of many diseases including Alzheimer’s disease, Parkinson’s disease, diabetes, rheumatoid arthritis and neurodegeneration, the use of antioxidants in pharmacology is intensively studied. Antioxidants are also widely used as dietary supplements and in industry as preservatives in food, cosmetics, rubber and gasoline. Simple, direct and high-throughput assays for total antioxidant capacity (TAC) find wide applications in research, food industry and drug discovery.
