The PolyATtract System isolates messenger RNA directly from total RNA.
- Isolates Highly Pure mRNA from Total RNA
- Protocol complete in approximately 45 minutes
- Isolated mRNA is suitable for use in in vitro translation, RT-PCR and cDNA synthesis
- Cat.# Z5200 and Z5210 contain sufficient reagents for 3 separate mRNA isolations, each from 1-5mg total RNA
- Cat.# Z5300 and Z5310 contain sufficient reagents for 15 separate mRNA isolations, each from 100-1,000µg total RNA
The PolyATtract mRNA Isolation Systems use the MagneSphere technology to isolate mRNA rapidly and effectively from total RNA. The systems use a biotinylated oligo(dT) primer to hybridize, at high efficiency in solution, to the 3' poly(A)+ region present in most mature eukaryotic mRNAs. The hybrids are bound to streptavidin coupled to paramagnetic particles, captured using a magnetic separation stand and washed at high stringency. The mRNA is eluted from the solid phase by the simple addition of ribonuclease-free, deionized water. With total RNA as the starting material, poly(A)+ mRNA is isolated in approximately 45 minutes. The isolated mRNA is suitable for all molecular biology applications, including in vitro translation and cDNA synthesis. Cat.# Z5200 contains sufficient reagents for 3 separate mRNA isolations, each from 1-5mg of total RNA. Cat.# Z5210 contains the same reagents as Cat.# Z5200, excluding the Magnetic Separation Stand. Cat.# Z5300 contains sufficient reagents for 15 separate mRNA isolations, each from 100-1,000ug of total RNA. Cat.# Z5310 contains the same reagents as Cat.# Z5300, excluding the Magnetic Separation Stand.
System II contains sufficient reagents for three separate mRNA isolations from 1–5 mg of total RNA each. System I contains the same reagents as System II, excluding the Magnetic Separation Stand. System III contains sufficient reagents for 15 separate mRNA isolations from 100–1000 μg of total RNA each. System IV contains the same reagents as System III, excluding the Magnetic Separation Stand.
The hybrids are bound to streptavidin coupled to paramagnetic particles, captured by a magnetic separation stand, and washed at high stringency. The mRNA is eluted from the solid phase by the simple addition of ribonuclease-free, deionized water. With total RNA as the starting material, poly(A)+ mRNA is isolated in approximately 45 minutes. The isolated mRNA is suitable for all molecular biology applications including in-vitro translation and cDNA synthesis.
Accessories information: Biotinylated Oligo(dT) Probe, 35 μL, available separately.
