Wheat Germ Extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors) and is used for in vitro translation..
- Components for Protein Synthesis
- Contains tRNA, ribosomes, initiation, elongation and termination factors
- Extract is cleared of endogenous amino acids, mRNA and plant pigments, minimizing background translation
Wheat Germ Extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors). Wheat Germ Extract is prepared by grinding wheat germ in an extraction buffer followed by centrifugation to remove cell debris. The supernatant is subjected to chromatography that separates endogenous amino acids and plant pigments from the extract. The extract also is treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum.
The extract is also treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum.
The supernatant is subjected to chromatography that separates endogenous amino acids and plant pigments from the extract.
