Dithiothreitol (DTT, Cleland's reagent) ≥99% for molecular biology
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
Synonyms:
(R*,R*)-1,4-Dimercapto-2,3-butandiol
DL-Dithiothreitol is a Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.
- Storage temperature: +4 °C
DL-Dithiothreitol is used for stabilizing sulfhydryl containing enzymes. It is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.
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Formula:
C₄H₁₀O₂S₂ MW: 154.25 g/mol Melting Pt: 40…50 °C Flash Pt: >110 °C (lit.) |
MDL Number:
MFCD00004877 CAS Number: 3483-12-3 EINECS: 248-531-9 |
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Specification Test Results
| Physical Appearance | White Powder |
| Half Life | 0.1 M KPO4 buffer (Half Life in hours):- pH 6.5 at 20 °C: 40, pH 7.5 at 20 °C: 10, pH 8.5 at 20 °C: 1.4, pH 8.5 at 0 °C: 11, pH 8.5 at 40 °C: 0.2, pH 8.5 at 20 °C with addition of 0.1 mM Cu2+: 0.6, pH 8.5 at 20 °C with addition of 1.0 mM EDTA: 4 |
| pH | (0.1 M in H2O, 25 °C): 4.0 to 6.0 (Lit.) |
| Solubility | Soluble in water (50 mg/mL - clear and colorless solution); soluble in ethanol, acetone, ethylate, chloroform and ether. |
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