HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid ≥98% cell culture reagent
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
Synonyms:
4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acid
HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. Its dissociation decreases as the temperature decreases which makes it a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
- Storage temperature: Room Temperature, Desiccate
HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. It is useful in the pH range 6.8-8.2.
Formula:
C₈H₁₈N₂O₄S MW: 238.31 g/mol Melting Pt: 234 °C (453 °F) (lit.) |
MDL Number:
MFCD00006158 CAS Number: 7365-45-9 EINECS: 230-907-9 |
Specification Test Results
Physical Appearance | White Powder |
UV/Visible Absorbance |
OD250 (10% aq soln) |
pH | 4.0 - 5.5 (1% aq soln) useful pH range 6.8 - 8.2 |
pKa | 7.55 at 25 °C (Lit.) |
Solubility | Soluble in water giving clear, colorless solution (10% aq soln). |
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