HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid ≥98% cell culture reagent

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

Synonyms: 4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acid

0219454990 0219454983 0219454980
IC0219454990EA 497.5 USD
IC0219454990 IC0219454980 IC0219454983
HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid ≥98% cell culture reagent
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)

HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. Its dissociation decreases as the temperature decreases which makes it a more effective buffering agent for maintaining enzyme structure and function at low temperatures.


  • Storage temperature: Room Temperature, Desiccate


HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. It is useful in the pH range 6.8-8.2.

Formula: C₈H₁₈N₂O₄S
MW: 238.31 g/mol
Melting Pt: 234 °C (453 °F) (lit.)
MDL Number: MFCD00006158
CAS Number: 7365-45-9
EINECS: 230-907-9
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Specification Test Results

Physical Appearance White Powder
UV/Visible Absorbance OD250 (10% aq soln) 0.2 , OD260 (10% aq soln) 0.1 and OD280 (10% aq soln) 0.1
pH 4.0 - 5.5 (1% aq soln) useful pH range 6.8 - 8.2
pKa 7.55 at 25 °C (Lit.)
Solubility Soluble in water giving clear, colorless solution (10% aq soln).

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