IPTG (isopropyl-β-D-thiogalactopyranoside) ≥99% for molecular biology

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

Synonyms: (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triolIPTGIsopropyl Beta-D-1-thiogalactopyranoside

0219402995 0219402980 0219402991 0219402990 0219402994
IC0219402990EA 57.93 USD
IC0219402990 IC0219402980 IC0219402991 IC0219402994 IC0219402995
IPTG (isopropyl-β-D-thiogalactopyranoside) ≥99% for molecular biology
IPTG (Isopropyl-β-D-thiogalactopyranoside)

Isopropyl b-D-1-thiogalactopyranoside is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli. It functions by binding to the lac repressor and altering its conformation. This inactivation prevents the repression of the b-galactosidase coding lac gene.


  • Storage temperature: -20 °C, desiccate


Isopropyl b-D-1-thiogalactopyranoside is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal. It is commonly used in cloning procedures that require induction of β-galactosidase activity. It is also used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Formula: C₉H₁₈O₅S
MW: 238.31 g/mol
Melting Pt: 105…115 °C
MDL Number: MFCD00063273
CAS Number: 367-93-1
EINECS: 206-703-0

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Specification Test Results

Physical Appearance White Powder
Optical Rotation (c=1, water)-26 ° to -33 °
pH 5.0 - 7.0 (4% aq soln)
Solubility Soluble in methanol and water (50 mg/mL - clear, colorless solution).
Sterilization of Solutions Solutions can be sterile filtered using a 0.2 mm filter.
Typical Working Concentration The final concentration typically used in indicator plates is 0.2 mM.

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