T4 DNA Polymerase, Promega

Supplier: Promega Corporation

T4 DNA Polymerase, Promega

T4 DNA Polymerase, Promega

T4 DNA Polymerase, Promega

T4 DNA Polymerase, Promega

M4211 M4215

PAM4211EA 157.29 USD

PAM4211 PAM4215

T4 DNA Polymerase, Promega

Nucleic Acid Reagents Nucleic Acid Modification and Labelling

High-fidelity polymerase useful for mutagenesis reactions, 3'-overhang removal and 5'-overhang fill-in reactions.

Appplications: Labeling in vitro, Flushing Ends
Synthesizes DNA 5´→3´ from a Primed ssDNA Template
High fidelity enzyme of choice for applications where misincorporation is a concern
Flexible polymerase used in a variety of molecular applications; active in many Promega 1X restriction enzyme buffers
Heat inactivated by heating at 75°C for 10 minutes
Supplied at a concentration of 5-10u/microl

T4 DNA Polymerase catalyzes the 5'-->3' synthesis of DNA from a primed single-stranded DNA template. Although possessing a potent 3'-->5' proofreading exonuclease, T4 DNA Polymerase contains no 5'-->3' exonuclease activity. T4 DNA Polymerase can be used to fill 5' protruding ends with labeled or unlabeled dNTPs or for the generation of blunt ends from DNA molecules with 3' overhangs.

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