DNA Polymerase I Large (Klenow) Fragment, Promega
Supplier: Promega Corporation
A DNA-dependent DNA polymerase that lacks the 5'-->3' exonuclease activity of intact E. coli DNA Polymerase I but retains its 5'-->3' polymerase, 3'-->5' exonuclease and strand displacement activities.
- Application: Labeling in vitro, Sequencing, Mutagenesis, cDNA Synthesis, Flushing Ends
- DNA-Dependent DNA Polymerase that Lacks the 5´→3´ Exonuclease Activity
- Use in a variety of molecular applications
- Heat-inactivate by heating at 75°C for 10 minutes
- Active in many Promega 1X restriction enzyme buffers
- Supplied at a concentration of 5u/microl
DNA Polymerase I Large (Klenow) Fragment is a DNA-dependent DNA polymerase that lacks the 5'-->3' exonuclease activity of intact E. coli DNA Polymerase I but retains its 5'-->3' polymerase, 3'-->5' exonuclease and strand displacement activities. The enzyme is a 68kDa C-terminal fragment of DNA Polymerase I. The 5'-->3' polymerase activity of Klenow Fragment can be used to fill in 5'-protruding ends with unlabeled or labeled dNTPs, to sequence single- or double-stranded DNA templates, for in vitro mutagenesis using synthetic oligonucleotides, for cDNA second-strand synthesis and to generate single-stranded DNA probes. The 3'-->5' exonuclease activity can be used to generate blunt ends from a 3'-overhang.
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