LymphoSep- Lymphocyte separation medium is a sterile, iso-osmotic, low viscosity solution for density centrifugation yielding a clear separation of lymphocytes from heparinized or defibrinated human blood.
- In vitro isolation of lymphocytes from diluted whole blood
- Maximum yield of mononuclear cells
- Easy one-step centrifugation
- >96% cell viability of lymphocytes
- Low endotoxins
- Autoclave sterilization
Ready to use quick, simple and reliable medium for the isolation of lymphocytes from whole blood. Lymphocytes are isolated by layering diluted, heparinized blood over a solution of sodium metrizoate that contains a polysaccharide. This polysaccharide aggregates the erythrocytes so that they can be sedimented by low centrifugation. The less dense lymphocytes remain as a clearly defined band at the plasma Lymphosep™ interface.
Delivery information: Lymphosep™ is supplied as a ready made sterile aqueous solution of sodium metrizoate (9.6% w/v) and Ficoll® (5.6% w/v).
Caution: Store at room temperature (15...30 °C), protect from light.