HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid free acid ≥99% (by titrimetric analysis on dry substance), ULTROL®, Calbiochem®, Millipore®

Supplier: MilliporeSigma

Synonyms: 4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acidN-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acidN-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acidN-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulphonic acid)N-2-Hydroxyethylpiperazine-N′-2-ethanesulphonic acidHEPES free acid

M-Clarity™ quality level = MQ100

391338-5KG 391338-100GM 391338-500GM 391338-25GM 391338-25GM 391338-1KG 391338-5KG 391338-100GM 391338-500GM 391338-1KG
80058-228EA 134.08 USD
80058-228 CA80058-236 80058-236 CA80058-234 80058-234 CA80058-232 80058-232 CA80058-230 80058-230 CA80058-228
HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid free acid ≥99% (by titrimetric analysis on dry substance), ULTROL®, Calbiochem®, Millipore®
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)

A zwitterionic N-substituted aminosulfonic acid buffer. HEPES has its maximum buffering capacity very near the pH of mammalian tissue fluids (pKa of 7.31 at 37°C). Exhibits no metal binding and has a small temperature dependent ΔpKa of -0.014/°C.


  • Maximum buffering capacity
  • Useful in the pH 6.8 to 8.2 range
  • pKa (25 °C) 7.5


Absorbance (1.0 M, H₂O, 260 nm):≤0.05
Absorbance (1.0 M, H₂O, 280 nm): ≤0.04


Caution: Storage condition: OK to freeze

Formula: C₈H₁₈N₂O₄S
MW: 238.31 g/mol
Storage Temperature: Ambient
MDL Number: MFCD00006158
CAS Number: 7365-45-9
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Specification Test Results

Assay ≥99% dry basis (titration)
Form Crystalline powder
Chloride (Cl-) ≤0.01%
Sulfate (SO42-) ≤0.05%
Color White

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