HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid free acid ≥99% (by titrimetric analysis on dry substance), ULTROL®, Calbiochem®, Millipore®
Supplier: MilliporeSigma
Synonyms:
4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid
, 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid
, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
, 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acid
, N-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid
, 4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acid
, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulphonic acid)
, N-2-Hydroxyethylpiperazine-N′-2-ethanesulphonic acid
, HEPES free acid
M-Clarity™ quality level = MQ100
A zwitterionic N-substituted aminosulfonic acid buffer. HEPES has its maximum buffering capacity very near the pH of mammalian tissue fluids (pKa of 7.31 at 37°C). Exhibits no metal binding and has a small temperature dependent ΔpKa of -0.014/°C.
- Maximum buffering capacity
- Useful in the pH 6.8 to 8.2 range
- pKa (25 °C) 7.5
Absorbance (1.0 M, H₂O, 260 nm):≤0.05
Absorbance (1.0 M, H₂O, 280 nm): ≤0.04
Caution: Storage condition: OK to freeze
Formula:
C₈H₁₈N₂O₄S MW: 238.31 g/mol Storage Temperature: Ambient |
MDL Number:
MFCD00006158 CAS Number: 7365-45-9 |
Specification Test Results
Assay | ≥99% dry basis (titration) |
Form | Crystalline powder |
Chloride (Cl-) | ≤0.01% |
Sulfate (SO42-) | ≤0.05% |
Color | White |
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