Self-Quenched BODIPY FL Conjugate of BSA is an ideal reagent to monitor lysosome function, the lysosome cargo degradation, or assess proteolytic activity in vitro.
Self-Quenched BODIPY FL Conjugate of BSA (Green), an ideal reagent to monitor lysosome function, the lysosome cargo degradation, or assess proteolytic activity in vitro.
Ideal reagent to monitor lysosome function
Insensitive to pH from pH 3-11
Narrow excitation and emission spectral bandwidths
Lyophilized from approximately 10 mg/mL protein in PBS. Reconstitute each vial in 100 µL water or PBS for a 10 mg/mL concentration. The suggested final concentration for cell culture applications is 10-20 µg/mL. The lyophilized product is stable for over six months at -20°C when stored desiccated and protected from light. Store the reconstituted aliquots at –20°C or –80°C.
The lysosome function/proteolytic activity can be detected with this conjugate by fluorescence microscopy, confocal microscopy, FACS analysis and fluorescence microplate reader. The extremely low background fluorescence of this conjugate and its high sensitivity to digestion by various proteases renders it particularly valuable in the imaging of extracellular or intracellular proteolytic activity. This conjugate has the advantage over fluorescein conjugates in being insensitive to pH from pH 3-11 and having narrow excitation and emission spectral bandwidths. The pH insensitivity allows the direct detection of proteolytic activity in situations where the pH is unknown and cannot be controlled or where the pH is known to be low, for example within lysosomes and endosomes.
