Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose beads. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with goat, horse, human, rabbit, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, virus titration assay, FRET-based assays, and small animal imaging.
Western blot: 1:5000
Type: Secondary
Antigen: IgG
Clonality: Polyclonal
Clone:
Conjugation: IRDye 800CW
Epitope:
Host: Goat
Isotype:
Reactivity: Mouse