This antibody was isolated from antisera by affinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule chicken IgY, and with the light chains of other chicken immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, goat, guinea pig, Syrian hamster, horse, mouse, human, rabbit, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ assay applications. Highly Recommended for: Western blot, In-Cell Western Assay, On-Cell Western Assay, protein array, immunohistochemistry, microsciopy, 2D gel detection, tissue section imaging, and small animal imaging.
Western blot: 1:15000; In-Cell Western Assay: 1:800
Type: Secondary
Antigen: IgG
Clonality: Polyclonal
Clone:
Conjugation: IRDye 680RD
Epitope:
Host: Donkey
Isotype:
Reactivity: Chicken