The antibody was purified from antisera by affinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG and with the light chains of other rat immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse, bovine, horse, human, and rabbit serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications. Recommended for: Western blot, proein array, immunohistochemistry, microscopy, 2D gel detection, and tissue section imaging. NOT RECOMMENDED for: small animal imaging, In-Cell Wesern assays, or On-Cell Western assays.
Western blot: 1:20000
Type: Secondary
Antigen: IgG
Clonality: Polyclonal
Clone:
Conjugation: IRDye 680LT
Epitope:
Host: Goat
Isotype:
Reactivity: Rat