GoTaq® qPCR Master Mix, Promega
Supplier: Promega Corporation
GoTaq qPCR Master Mix is a ready-to-use, stabilized 2X formulation containing GoTaq(R) Hot Start Polymerase, MgCl2, dNTPs, BRYT Green(R) Dye and a proprietary reaction buffer.
- Efficient qPCR for Sensitive Amplification
- Ready-to-use mix of optimized components for robust amplification
- Compatible with both fast and standard cycling methods
- Resistant to a wide range of PCR inhibitors
The GoTaq qPCR Master Mix is optimized for fast and reproducible quantitative PCR assays using a double-stranded DNA-binding dye. The master mix contains BRYT Green dye, a novel fluorescent DNA-binding dye with minimal PCR inhibition for maximum PCR efficiency and greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR Green I. The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq Hot Start Polymerase, MgCl2, dNTPs, BRYT Green Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.
The master mix contains BRYT Green® dye, a novel fluorescent DNA-binding dye with minimal PCR inhibition for maximum PCR efficiency and greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR® Green I.
It's optimized for fast and reproducible quantitative PCR assays using a double-stranded DNA-binding dye.
Caution: Store all components at –30 to –10°C upon their arrival, and protect them from light. To use components immediately, store them at 2 to 8°C, protected from light, for up to three months.
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