Pierce™ TMTzero™ Protein Quantitation Reagent, Thermo Scientific

Supplier: Thermo Fisher Scientific
Pierce
90067 90067
PI90067EA 318.46 USD
CAPI90067 PI90067
Pierce™ TMTzero™ Protein Quantitation Reagent, Thermo Scientific
Protein Extraction Protein Extraction Reagents
Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling

  • Used in mass spectrometry as a tag for MS-level peptide quantitation
  • Amine-reactive
  • Enables identification and quantitation of proteins

Tandem Mass Tag (TMT) Reagents are chemical tags that enable identification and quantitation of proteins in different samples using tandem mass spectrometry. The amine-reactive, NHS-ester-activated compounds covalently attach to the peptide amino terminus and free amino termini of lysine residues of peptides and proteins with high efficiency, thereby labeling all peptides in a given sample regardless of enzyme used for digestion. Since TMT reagents share an identical structure and mass (i.e., isotopomers), labeled peptides co-elute during LC separation and are co-isolated during MS/MS analysis, resulting in fewer missing peptide identifications among samples. During MS/MS analysis, each isobaric tag is also fragmented to produce a unique reporter ion mass that is used for sample identification and quantitation. Protein quantitation is accomplished by comparing the relative intensities of the two reporter ions in the MS/MS spectra.

Store at -20°C.

Caution: For Research Use Only. Not for use in diagnostic procedures.
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