Pierce™ HisPur™ Ni-NTA Spin Plates, Thermo Scientific

Supplier: Thermo Scientific
Pierce Pierce™
88230 88230
PI88230EA 572.17 USD
CAPI88230 PI88230
Pierce™ HisPur™ Ni-NTA Spin Plates, Thermo Scientific
Microplates Filter Plates
HisPur™ Ni-NTA Spin Plates contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins

  • High capacity bind up to 60 mg of 6xHis-tagged protein per mL of resin
  • Purify proteins using native or denaturing conditions
  • Use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
  • Reuse the same batch of resin at least five times
  • Available in multiple formats including bulk resin, spin columns, chromatography cartridges and 96-well filter plates

HisPur™ Ni-NTA Resin and HisPur™ Cobalt Resin are alternative forms of IMAC, the former using nickel and the latter using cobalt as the chelated metal ion responsible for His-tag binding. Ni-NTA resins have been the most common IMAC resin choice for 6xHis-tag protein purifications because of the four metal-binding sites on the chelate, which enables high-protein binding and low-metal ion leaching. Cobalt binds less strongly and therefore more discriminantly, enabling greater purity but usually less yield in His-tagged protein purification.

Caution: For Research Use Only. Not for use in diagnostic procedures.It should be stored at 4˚C
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