5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5/6-FAM SE) ≥90% (by HPLC) fluorescent dye, Pierce™

Supplier: 0000013509

Synonyms: 5(6)-FAM SENHS-FluoresceinFLUOS

46410 46410 46409 46409
PI46410EA 147.32 USD
PI46410 CAPI46410 PI46409 CAPI46409
5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5/6-FAM SE) ≥90% (by HPLC) fluorescent dye, Pierce™
5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5/6-FAM SE)

NHS fluorescein [(5/6-carboxyfluorescein succinimidyl ester), mixed isomer] is an amine reactive derivative of fluorescein dye for antibodies and other probes in fluorescence microscopy, flow cytometry, and immunofluorescence based assays such as western blotting and ELISA.


  • Label antibodies for use as immunofluorescent probes
  • Label oligonucleotides for hybridization probes
  • Detect proteins in gels and on Western blots


NHS fluorescein is activated with the N-hydroxy-succinimidyl-ester (NHS ester) functional group. These fluorescein dyes are mixtures of isomers with reactive groups attached at the 5 and 6 positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer.


Caution: For Research Use Only. Not for use in diagnostic procedures.

Formula: C₂₅H₁₅NO₉
MW: 473.39 g/mol
MDL Number: MFCD04119713
CAS Number: 117548-22-8

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