Thermo Scientific SulfoLink Immobilization Kit for Proteins is a kit containing all the reagents for preparing the protein sample for efficient immobilization and five columns containing SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.
- Specific to sulfhydryl (-SH) groups
- Fast—prepare and couple protein samples in 3.5 hours
- Flexible coupling conditions, use pH 7.5 to 9.0 aqueous buffers, organic solvent or denaturant
- Streamlined protocols for sample preparation, immobilization, and affinity purification
- High capacity—Immobilize 2 to 20 mg of protein per 2 mL column of SulfoLink Coupling Resin
When incubated with a solution of protein that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the protein to the resin
Applications:
Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification
The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.
Delivery information: Kit contains SulfoLink Columns, 2 mL (5 columns), SulfoLink Preparation Buffer (7.5 mL), SulfoLink Coupling Buffer (500 mL), Wash Solution (120 mL), PBS Pack (makes 500 mL), 2-Mercaptoethylamine?HCl (5 x 6 mg), L-Cysteine?HCl (100 mg), Zeba Spin Desalting Columns, 5 mL (5 columns), and Column Accessories.
Caution: For Research Use Only. Not for use in diagnostic procedures. Store at 4˚C protected from light.
