Pierce™ Polyacrylamide Desalting Columns, Thermo Scientific

Supplier: Thermo Scientific
Pierce
89862 43426 89849 43243 43426 43243 89862
PI43243EA 188.99 USD
PI43243 CAPI43243 CAPI89862 PI89849 PI43426 CAPI43426 PI89862
Pierce™ Polyacrylamide Desalting Columns, Thermo Scientific
Chromatography Columns
Protein desalting spin columns are designed to desalt or exchange buffer of protein samples with volumes of 30 to 120 µL. These devices have exceptional desalting characteristics with ≥95% retention of salts and small molecules while providing excellent recovery of proteins greater than 7 kDa. Multiple samples can be processed in less than 5 minutes without cumbersome column preparation steps.

  • Stable in water, salt solutions, organic solvents and alkaline or acidic conditions
  • Excellent flow properties
  • Heat-stable

These columns are pre-packed with porous polyacrylamide beads and provide for buffer-exchange and desalting of proteins and other macromolecular samples based on molecular-weight cutoff of 7 kDa

The polyacrylamide resin is not subject to enzymatic degradation and will not serve as a nutrient for microbial growth. The resin is very hydrophilic, thereby minimizing undesirable binding interactions between the resin and sample molecules.

The resin is susceptible to hydrolysis of amide groups under extreme pH conditions, so an operating pH of 2 to 10 is recommended at room temperature. The polyacrylamide resin can also be autoclaved at pH 5.5 to 6.5 for 30 minutes at 120 °C.

It's ideal applications include, removing salts from protein solutions, phenol from nucleic acid preparations, separating excess crosslinker from conjugate preparations, removing excess derivatizing agents from modified proteins, unreacted dye from fluorescent antibodies, free radiolabel from labeled proteins and exchanging one buffer for another.

Caution: For Research Use Only. Not for use in diagnostic procedures. Upon receipt store at room temperature or 4˚C. Product shipped at ambient temperature.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR