Pierce EZ-Link® Carboxyl Reactive Biotinylation Reagents, Thermo Scientific

Supplier: Thermo Fisher Scientific
EZ-Link®
26139 26139 21345 26137 21347 21347 21346 26137 21345 26136 26136 21346
PI26139EA 510.57 USD
CAPI21347 PI26139 PI21347 PI26137 PI21346 PI21345 CAPI26137 PI26136 CAPI26136 CAPI21346 CAPI26139 CAPI21345
Pierce EZ-Link® Carboxyl Reactive Biotinylation Reagents, Thermo Scientific
Protein Linking and Labeling Systems
Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatized biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4.5 - 5 and requires just minutes to complete.

Buffers containing primary amines (tris, glycine, etc.) or carboxyls (acetate, citrate, etc.) must be avoided because they will quench the reaction

Pentylamine-Biotin: Dissolves in aqueous solutions. Use the EDC crosslinker to couple this analogue to carboxyl (–COOH) groups. Coupling with EDC occurs at pH 4 - 6.

Amine-PEGn-Biotin: Water soluble, PEG-containing reagents with terminal primary amines (–NH2). The PEG spacer arm is hydrophilic and confers greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers. The amine group of these reagents can be reacted with carboxyl groups on carboxy termini, aspartate residues or glutamate residues using EDC.

Alkoxyamine-PEG-Biotin: Used for biotinylating macromolecules at carbohydrate groups that have been oxidised to form aldehydes. The alkoxyamine group reacts to carbonyls (aldehydes and ketones), resulting in an oxime linkage. Alkoxyamine-PEG-Biotin reagents contain a multi-functional extended spacer arm that is a flexible, non immunogenic hydrophilic polyethylene glycol (PEG), which imparts water solubility to labeled molecules. Consequently, antibodies labeled with biotin alkoxyamine reagents exhibit less aggregation when stored in solution compared to antibodies labeled with reagents having only hydrocarbon spacers.

Caution: For Research Use Only. Not for use in diagnostic procedures.
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