Thiocarbohydrazide ≥99%, highest purity
Supplier: Electron Microscopy Sciences
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Danger
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Synonyms:
TCH
Thiocarbohydrazide is an osmiophilic reagent. It is used in the OTO staining method (osmium fixed tissue exposed to TCH followed by a second treatment with osmium) to enhance the contrast of all osmophilic components of the cell, especially lipids, which hold the most osmium.
- 99% minimum purity - NH 2 NHCSNHNH 2
- Pure white crystalline form M.P. is 172°C
- Indefinite shelf-life due to purification procedure
- Does not need to be kept refrigerated
Seligman A.M. et al (1965).Histochemical demonstration of some oxidized macromolecules with thiocarbohydrazide (TCH) or thiosemicarbazide (TSC) and osmium tetroxide. J. Histochem. Cytochem., 13:629
Seligman, A.M. et al (1966). A new staining method (OTO) for enhancing contrast of lipid-containing membranes and droplets in osmium tetroxide fixed tissue with osmiophilic thiocarbohydrazide (TCH). J. Cell Biol., 30:424
Thiery, J.P. (1967). Mise en evidence des polysaccharides sur coupes fines en microscopie electronique. J. Microsc., 6:987
Lo, H.K. et al (1987). A modified periodic acid-thiocarbo-hydrazide-silver proteinate staining sequence for enhanced contrast and resolution of glycogen depositions by transmission electron microscopy. J. Histochem. Cytochem., 35:393
Neiss, W.F. (1988). Enhancement of the periodic acid-Schiff (PAS) and periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) reaction in LR White sections. Histochemistry, 88:603
Hanker, J.S. et al (1964). Osmiophilic reagents: new cytochemical principles for light and electron microscopy. Science, N.Y., 146:1039
Derenzini, M., et al (1986). An improved periodic acid-thiosemicarbazide-osmium technique to reveal glyco-conjugates at the molecular level in situ. J. Histochem. Cytochem.,34:1161"
Formula:
CH₆N₄S MW: 106.15 g/mol Storage Temperature: Ambient |
MDL Number:
MFCD00007616 CAS Number: 2231-57-4 UN: 2811 ADR: 6.1,II |
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