Anti-AGO2 Mouse Monoclonal Antibody [clone: 2A8]

Supplier: Rockland Immunochemicals
200-301-V04 200-301-V04
10799-598EA 528 USD
CA10799-598 10799-598
Anti-AGO2 Mouse Monoclonal Antibody [clone: 2A8]
Antibodies
Members of the Argonaute (Ago) protein family are internal to RISC function. Argonaute proteins bind to mature microRNA (miRNAs) and short interfering RNAs (siRNAs) and form the core components of effector complexes that mediate miRNA and siRNA function. Argonautes are needed for miRNA-induced silencing and contain two conserved RNA binding domains: a PAZ domain that can bind the single stranded 3’ end of the mature miRNA and a PIWI domain that structurally resembles ribonuclease-H and functions to interact with the 5’ end of the guide strand. Some argonautes, for example human Ago2, cleave target transcripts directly; argonautes may also recruit additional proteins to achieve translational repression. The human genome encodes eight Argonaute proteins divided by sequence similarities into two families: AGO (with four members present in all mammalian cells and called E1F2C/hAgo in humans), and PIWI (found in the germ line and hematopoietic stem cells). Anti-Ago Antibody is ideal for research in Alternative Splicing, Gene Expression and Gene Silencing.

Recommended Dilutions: Immunohistochemsitry: 1:500; Immunofluorescence Microscopy: 1:500; Immunoprecipitation: 8 ?g; Western Blot: 1:300 - 1:500; contains 0.05% (w/v) Sodium Azide

Type: Primary
Antigen: AGO2
Clonality: Monoclonal
Clone: 2A8
Conjugation: Unconjugated
Epitope: Center
Host: Mouse
Isotype:
Reactivity: Human, Mouse, Other (Wide Range)
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