NHS-Activated Sepharose™ 4 Fast Flow Affinity Coupling Media, Cytiva

Supplier: Cytiva
Sepharose™
17-0906-01 17-0906-01 17-0906-01
97067-756EA 385.1 USD
97067-756 95017-030 CA95017-030
NHS-Activated Sepharose™ 4 Fast Flow Affinity Coupling Media, Cytiva
Protein Purification Protein Purification Resins
NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.

  • For affinity chromatography
  • NHS pre-activated medium for coupling of small amino-containing proteins and peptides in process-scale applications
  • NHS coupling method results in chemically stable ligand attachment
  • BioProcess medium supported for industrial applications
  • High level of pre-activation gives a high degree of substitution of the selected ligand
  • Stable in commonly used aqueous buffers provided that the ligand withstand the pH or the chemical environment

NHS-activated Sepharose 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, NHS-activated Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support. This resin provides a spacer arm to the coupled ligand and is therefore particularly suitable for immobilising smaller protein and peptide ligands.

NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups.
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