The T7 RiboMAX Express RNAi System is an in vitro transcription system designed to produce milligram amounts of RNA suitable for use in RNAi assays.
- Produces Milligam Amounts of RNA for RNA Interference Experiments
- siRNA duplexes for mammalian RNAi
- Long dsRNA for nonmammalian RNAi
- Produces milligram amounts of double-stranded RNA in 30 minutes
The T7 RiboMAX Express RNAi System is an in vitro transcription system designed for producing milligram amounts of double-stranded RNA (dsRNA) in a short amount of time. The dsRNA is free of protein and other contaminants and is suitable for use in RNA interference (RNAi) in both mammalian and nonmammalian systems. The T7 RiboMAX Express RNAi System can be used to synthesize short interfering RNAs (siRNAs) of 21bp for use in mammalian systems. siRNAs synthesized in vitro have been demonstrated to be as effective as chemically synthesized siRNAs for inducing RNAi in mammalian cells. In addition, the T7 RiboMAX Express RNAi System can be used for the synthesis of dsRNA molecules of approximately 200bp or greater, which can be applied to nonmammalian systems. Two complementary RNA strands are synthesized from DNA template (either plasmid or PCR product). The resulting RNA strands are annealed after the transcription reaction to form dsRNA. Any remaining single-stranded RNA and DNA template are removed with a nuclease digestion step. The dsRNA is then purified by isopropanol precipitation and can be introduced into the organism of choice for RNAi applications.
Two complementary RNA strands are synthesized from DNA template (either plasmid or PCR product). The resulting RNA strands are annealed after the transcription reaction to form dsRNA. Any remaining single-stranded RNA and DNA template is removed with a nuclease digestion step. The dsRNA is then purified by isopropanol precipitation and can be introduced into the organism of choice for RNAi applications.
The T7 RiboMAX Express RNAi System can be used to synthesize short interfering RNAs (siRNAs) of 21 bp for use in mammalian systems. siRNAs synthesized in-vitro have been demonstrated to be as effective as chemically synthesized siRNAs for inducing RNAi in mammalian cells. In addition, the T7 RiboMAX Express RNAi System can be used for the synthesis of dsRNA molecules of approximately 200 bp or greater, which can be applied to nonmammalian systems.
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