HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid free acid ≥98%, white powder

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

Synonyms: 4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acidN-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulphonic acidN-2-Hydroxyethylpiperazine-N′-2-ethanesulfonic acid4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acidN-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulphonic acid)N-2-Hydroxyethylpiperazine-N′-2-ethanesulphonic acidHEPES free acid

0210192683 0210192690 0210192680 0210192694 0210192650 0210192625 0210192691
IC10192683EA 261.41 USD
IC10192683 IC10192694 IC10192650 IC10192680 IC10192691 IC10192690 IC10192625
HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid free acid ≥98%, white powder
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)

HEPES is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers.


HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. Its dissociation decreases as the temperature decreases which makes it a more effective buffering agent for maintaining enzyme structure and function at low temperatures.


It is useful in the pH range 6,8-8,2.


Soluble in water giving clear, colorless solution (10% aq soln).


Caution: Store Desiccated.

Formula: C₈H₁₈N₂O₄S
MW: 238.31 g/mol
Melting Pt: 234 °C (453 °F) (lit.)
Storage Temperature: Ambient
MDL Number: MFCD00006158
CAS Number: 7365-45-9
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Specification Test Results

Identity Test Passes
Purity ≥98%
OD250 (10% aq soln) <0.2
OD260 (10% aq soln) <0.1
OD280 (10% aq soln) <0.1
pH (1% aq soln) 4.0-5.5
Moisture <1.0%
Solubility (10% aq soln) Clear, Colorless Solution

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