Hematoxylin hydrate, orange powder C.I. 75290

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Hematoxylin hydrate, orange powder C.I. 75290
Hematoxylin hydrate

Hematoxylin is Gill’s Formulation 2; a nuclear counter stain of blue-violet color. It is extracted from the heartwood of the logwood tree.


When oxidized it forms haematein, a compound that forms strongly coloured complexes with certain metal ions, the most notable ones being Fe(III) and Al(III) salts. Metal-haematein complexes are used to stain cell nuclei prior to examination under a microscope. Structures that stain with iron- or aluminium-haematein are often called basophilic, even though the mechanism of the staining is different from that of staining with basic dyes.


Used as a nuclear stain in cytology and histology applications.


Soluble in ethanol (20 mg/ml); hot water.


Hematoxylin is Gill′s Formulation 2; a nuclear counter stain of blue-violet color. It is extracted from the heartwood of the logwood tree. When oxidized it forms haematein, a compound that forms strongly coloured complexes with certain metal ions, the most notable ones being Fe(III) and Al(III) salts. Metal-haematein complexes are used to stain cell nuclei prior to examination under a microscope. Structures that stain with iron- or aluminium-haematein are often called basophilic, even though the mechanism of the staining is different from that of staining with basic dyes.

Formula: C₁₆H₁₄O₆·xH₂O
Melting Pt: 229…231 °C (lit.)
Storage Temperature: Ambient
MDL Number: MFCD00078111
CAS Number: 517-28-2
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Specification Test Results

Identity Test Passes
UV/VIS: l max (meOH) 290 - 292 nm
Loss on Drying Report Result

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