Betaine, anhydrous, white powder for biochemistry
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
Synonyms:
TMG, Trimethylglycine, 2-(Trimethylammonio)acetate
Betaine is a quaternary amine found in bacteria and higher plants which acts as an osmoprotectant (able to restore and maintain osmotic balance in living cells) and enhances the freezing tolerance of of living organisms. This is probably due to its involvement in the protection of macrocomponents of plant cells, such as protein complexes and membranes, under stress conditions. Betaine is biosynthesized in living cells by a two-step oxidation method from choline. The primary enzyme involved in this biosynthesis is choline oxidase.
- Presentation: White Powder
Betaine is a reagent that is used in soldering, resin curing fluxes, and organic synthesis. Betaine has been studied in its role in protecting plants from environmental stress and in maintaining osmolarity in mammalian cells. Betaine has been reported to diminish the base pair composition dependence of DNA thermal melting transitions, with a concentration of 5.2 M betaine being the value at which AT and GC base pairs are equally stable and hence subsequently found application in the polymerase chain reaction (PCR). The usefulness of betaine in PCR has resulted from its ability to enhance DNA amplification by diminishing the formation of secondary structure in GC-rich DNA regions. A protocol for the production of long-chain cDNAs that uses betaine and trehalose has been published. The permanent positive charge on betaine makes it a target of study for mass spectrometry (MS) analysis by such methods as electrospray ionization (ESI) MS.
End-product of oxidative metabolism of choline, betaine is a general methyl donor, in particular in a minor pathway of methionine biosynthesis. It is used to treat homocystinuria, which is a defect in the major pathway of methionine biosynthesis.
End-product of oxidative metabolism of choline, betaine is a general methyl donor, in particular in a minor pathway of methionine biosynthesis. It is used to treat homocystinuria, which is a defect in the major pathway of methionine biosynthesis.
Soluble in water (50 mg/mL), yielding a clear,colorless solution to faint yellow solution, methanol (100 mg/ml - clear solution) and ethanol (8.7 g/100 g).
Caution: Research Use Only (RUO).
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Formula:
C₅H₁₁NO₂ MW: 117.15 g/mol Melting Pt: 310 °C Storage Temperature: Refrigerator |
MDL Number:
MFCD00012123 CAS Number: 107-43-7 |
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Specification Test Results
| Identity Test | Passes |
| FTIR | Conforms to Standard |
| Moisture | ≤1% |
| Bulk Density | 0.50g/ml |
| Tapped Density | 0.65g/ml |
| Cadmium | <0.3 ppm |
| Chromium | <2 ppm |
| Lead | <1 ppm |
| Mercury | <0.2 ppm |
| pH | 5.84 |
| Heavy Metals | <10 ppm |
| Arsenic | <1 ppm |
| Residue on Ignition | 0.08% |
| Assay | 98.1% |
| Total Plate Count | <1000 cfu/g |
| Coliform | <30 Mpn/100g |
| Yeast and Mold | <100 cfu/gm |
| E. coli, Salmonella, Staphylococcus aureus, and Shigella | Negative |
| Particle Size | 100% thru 20 mesh |
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