B834 Competent Cells, Expression Strains, MilliporeSigma

Supplier: MilliporeSigma
69041-4 69042-3 69042-4 69041-3
80030-048EA 419.92 USD
80030-048 80030-050 80030-052 80030-046
B834 Competent Cells, Expression Strains, MilliporeSigma
Competent Cells
A common method for transformation of plasmids into E

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilising pET recombinants encoding target proteins that affect cell growth and viability.

The standard volume per tube is 0,2 ml,More Product Information
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