The ImmEdge™ Hydrophobic Barrier Pen can be used to draw a barrier around a section on a slide that keeps aqueous solutions in place and minimizes the amount of reagent needed for each section.
- Heat stable
- Stable for use with buffers with and without detergent (Tween 20, Triton X-100, etc.)
- Keeps reagents localized on cells and tissue preparations
- Light blue barrier
- Compatible with enzyme or fluorescence-based detection systems
- Soluble in all commonly used clearing agents
- Environmentally friendly - does not contain "ozone-depleting" substances
The ImmEdge™ Pen is an improvement over the PAP pen for immunohistochemistry, immunocytochemistry, immunofluorescence, and in situ hybridization. It is designed to provide a heat-stable, water-repellent barrier that keeps reagents localized on tissue specimens and prevents mixing of reagents when multiple sections are mounted on the same slide. The ImmEdge™ Pen barrier is stable for use with buffers with and without detergent (e.g. Tween 20 or Triton X-100, etc.).
The pale blue barrier is insoluble in alcohol and acetone but is completely removed by all commonly used xylene and xylene substitute clearing agents. The ImmEdge™ Pen is compatible with enzyme or fluorescence-based detection systems.
The ImmEdge™ Pen is environmentally friendly. It does not contain "ozone-depleting" solvents as identified by the Montreal Protocol or the Council of the European Union (specifically, fluorocarbons and chlorocarbons).
