Reactive CF® Dyes and Protein Labeling Kits, Biotium

Supplier: Biotium

CF™
92050 92040 92106 92058 92038 92044 92219 92024 92052 92215 92131 92109 92026 92041 92036 92213 92155 92056 92211 92139 92228 92212 92127 92029 92128 92133 92222 92102 92045 92105 92132 92226 92108 92053 92035 92104 92130 96011 92065 92043 92023 92142 92046 92210 92135 92209 92021 92223 92137 92042 92216 92225 92033 92112 92120 96010 92110 92214 92208 92218 92037 92020 92022 92150 92030 92051 92032 92025 92134 92107 92217 92111 92059 92220 92027 92221 92028 92055 92054 92034 92031 96012 92057 92039
89410-694EA 364.73 USD
89410-694 10018-208 89410-692 89410-690 75832-536 89139-826 89410-810 10154-192 89139-828 89410-698 89139-822 89410-696 75832-534 89410-730 89139-824 89411-582 89139-830 89410-808 89411-584 89410-728 10154-194 89410-806 89410-726 89410-804 10001-654 89411-580 89411-586 89411-588 89410-684 89139-818 89410-724 89139-814 89410-802 89139-816 89410-722 75845-662 89410-800 89410-720 89410-688 89410-686 71004-314 89411-592 89139-862 89411-594 89139-820 89139-864 89410-718 89411-590 89410-716 75784-064 89411-596 75784-062 89410-794 89410-792 89410-790 10018-226 89410-714 89410-712 89139-844 89410-798 89410-710 89410-796 89427-114 89410-708 89410-706 89410-704 89411-600 89411-602 89411-566 89410-702 89139-836 89410-788 89139-838 89410-700 89410-786 89427-116 89139-832 89139-840 89411-572 89139-842 89411-578 89427-082 89411-574 89411-576
Reactive CF® Dyes and Protein Labeling Kits, Biotium
Protein Linking and Labeling Systems
Biotium offers a number of reactive dye formats for labelling proteins, nucleic acids, and other biomolecules with fluorescent dyes. CF® dyes offer advantages in brightness and photostability compared other similar commercial dyes. CF® dyes are available in a number of reactive dye formats for CF® dye colours span the visible and near-infrared spectra.

Amine groups react with activated carboxylic acids. Aminooxy (also known as hydroxylamine) groups readily react with aldehydes or ketones without the need for a reducing agent. Hydrazides can be microinjected into cells as fixable polar tracers for visualization of neuronal morphology. Hydrazides also can be used for labeling aldehyde or ketone groups, such as carbohydrate molecules after peroxidation with periodate. However, for this application we recommend using CF dye aminooxy forms, which are more reactive toward these groups than hydrazide forms. Maleimide reactive dyes label thiols, such as cysteine residues in proteins, and can be used as an alternative to SE labeling in cases where lysine modification alters the biological activity of the labeled protein. Succinimidyl ester (SE) groups label free amines, such as lysine residues in proteins, and are commonly used to label antibodies. CF® dye SE groups are more resistant to hydrolysis compared to most commercially available SE dyes, resulting in improved stability and labeling efficiency. SE Protein Labeling Kits include CF® dye SE and other components required to label and purify proteins. The kits are sufficient for three labeling reactions of 1 mg protein each.

CF® dye technologies are covered by pending US and international patents. CF® is a registred trademark of Biotium, Inc. AlexaFluor® is a registered trademark of Molecular Probes, Inc.

Biotium's line of CF® dyes offer advantages in brighness and/or photostability compared to Alexa Fluor® dyes and other similar commercial dyes. CF™ dyes are available in a number of reactive dye formats for CF™ dye colors span the visible and near-infrared spectra. See the CF® Dye Reference Table under the Datasheets tab to select the dye colors that are optimal for your application and instrument.

Ordering information: Succinimidyl Ester, Maleimide, and SE Protein Labeling Kits include a detailed protocol for labeling IgG or other proteins. See also: Mix-n-Stain™ Antibody Labeling Kits for rapid conjugation of 5 to 100 µg antibody.
Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR