HisTrap™ Excel Affinity Chromatography Columns

Supplier: Cytiva
Histrap™
17371205 29-0485-86 17371206 17371205 17-3712-06 29-0485-86 17371206 17-3712-05 17371205
89501-372EA 91.92 USD
89501-372 CA89233-414 89233-412 CA89233-412 89233-414 CA89234-482 89234-480 CA89234-480 CA11033-012
HisTrap™ Excel Affinity Chromatography Columns
Chromatography Columns
HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • HisTrap excel columns allow direct purification of cell-free, unclarified samples

HisTrap excel 1 ml and 5 ml are ready-to-use IMAC columns prepacked with Ni Sepharose excel. The design of the columns in combination with the specific properties of the resin enables fast and convenient purifications.

The special type of filter in the top and bottom of the columns makes it possible to load large volumes of cell-free, unclarified samples directly on the columns without causing back pressure problems. This time-saving property helps prevent degradation and loss of sensitive target proteins.

HisTrap excel columns are made of biocompatible polypropylene.

HisTrap excel columns can be operated either with a peristaltic pump or with chromatography systems such as ÄKTA systems. The columns cannot be opened or refilled.

Delivery information: Supplied with a stopper on the inlet and a snap-off end on the outlet.
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