Affi-Gel and Affi-Prep Protein A Media, Bio-Rad

Supplier: BIO-RAD LABORATORIES MS

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101642-986EA 1863.08 USD
101642-986 101643-006 101642-980 101642-982 101643-004 101642-978
Affi-Gel and Affi-Prep Protein A Media, Bio-Rad
Protein Purification Protein Purification Resins

Chromatography on Affi-Gel and Affi-Prep protein A media yields highly purified immunoglobulins (IgG), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification


  • High purity of IgGs
  • High affinity for mammalian IgG
  • High capacities for mouse IgG1 as well as other subclasses with MAPS optimized buffer


With Affi-Gel and Affi-Prep protein A media and MAPS II buffers, you can purify up to 10 mg of IgG1/mL of gel. This is 8–10 times higher than with standard methods. In addition, the MAPS process and Affi-Gel protein A permit greater binding of mouse IgG1 than do immobilized protein G media.


Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species. In addition, Affi-Prep media offer high linear flow rates up to 2,000 cm/hr, pressure stability up to 1,000 psi (70 bar), and high chemical stability, which allows sanitization with 0.1 M NaOH.

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