You Searched For: Protein Extraction Kits

Catalog Number: (82022-696)

Supplier: G-Biosciences

Description: G-Biosciences' FOCUS™ PhosphoRich™ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples

Catalog Number: (82021-380)

Supplier: G-Biosciences

Description: G-Biosciences' InGel™ Array is a 96-well format kit suitable for processing larger numbers of protein spots concurrently and is compatible with spot-picking instruments

Catalog Number: (89167-892)

Supplier: G-Biosciences

Description: G-Biosciences' InGel™ Blue kit provides a complete set of reagents for the in gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS)

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Catalog Number: (PAL4151)

Supplier: Promega Corporation

Description: Untreated Rabbit Reticulocyte Lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors) but has not been treated with micrococcal nuclease.

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Catalog Number: (82022-684)

Supplier: G-Biosciences

Description: G-Biosciences' FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.

Supplier: Avanti Polar Lipids Inc

Description: Polar lipid extract is derived from the total lipid extract by precipitation with acetone followed by extraction of the acetone insoluble material with diethyl ether.

Catalog Number: (PI84868)

Supplier: Invitrogen

Description: Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.Easy-to-use—resin provided in single-use spin column formatImproved protein identifications—protein identifications increased by ≥50% when compared to unfractionated samplesReproducible—elution profiles and fractional resolution vary by less than 20%Optimized—robust procedure for maximal protein identification and peptide recovery while minimizing fractional overlapCompatible—reagents validated with a variety of complex samples, including TMT™-labeled peptidesIn order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis. The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-phase chromatography to separate peptides by hydrophobicity and provides excellent orthogonality to low pH reversed-phase LC-MS gradients.The kit has been designed to improve protein identification through the use of a proprietary reversed-phase resin in an easy-to-use spin column format with a highpH fractionation protocol. In contrast to strong cation exchange (SCX) fractionation, the high-pH reversed-phase fractions do not require an additional desalting step before LC/MS analysis.The High pH Reversed-phase Peptide Fractionation Kit includes a high pH buffer (0.1% triethylamine) and twelve spin columns containing pH-resistant reversed-phase resin. Each reversed-phase fractionation spin column enables fractionation of 10–100 µg of peptide sample using a microcentrifuge.Native, phosphorylated, Tandem Mass TagTM (TMTTM)-labeled, and other complex peptide mixture samples can be fractionated using this kit. Combining the search results generated by the individual fractions helps improve protein sequence coverage and increases the number of identified proteins relative to unfractionated samples.Applications:Reducing sample complexity to identify targets of interestConducting systems biology studiesReducing sample complexity to improve quantitation studies

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Catalog Number: (PI89826)

Supplier: Invitrogen

Description: The Mem-PER™ Eukaryotic Membrane Protein Extraction Kit enables small-scale solubilization and enrichment of hydrophobic membrane-associated proteins in a simple reagent-based procedure

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Catalog Number: (PI89881)

Supplier: Invitrogen

Description: The Pierce™ Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins to the exclusion of intracellular proteins.

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Catalog Number: (101644-016)

Supplier: Bio-Rad

Description: The ReadyPrep protein extraction kit (membrane II) applies sodium carbonate extraction methods (Fujiki et al

Catalog Number: (PI88279)

Supplier: Invitrogen

Description: The Pierce™ Mouse Embryonic Fibroblast Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of fibroblasts from mouse embryos.

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Catalog Number: (101644-026)

Supplier: Bio-Rad

Description: The ReadyPrep protein extraction kit (cytoplasmic/nuclear) prepares fractions enriched in cytoplasmic and nuclear proteins from eukaryotic samples, such as cultured cells and tissues.

Supplier: Invitrogen

Description: Imject™ Mariculture KLH (mcKLH) is a purified keyhole limpet haemocyanin carrier protein that enables simple preparation of highly effective immunogens with peptide antigens.

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Catalog Number: (PI89875)

Supplier: Invitrogen

Description: The Pierce™ Albumin/IgG Removal Kit is optimised to decrease the abundant albumin and antibody components of human serum samples in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.

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Supplier: Invitrogen

Description: The Pierce™ Rapid Isotyping Kit is a 5-minute lateral-flow assay with ELISA sensitivity for class and subclass determination of monoclonal antibodies.

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Catalog Number: (PI89874)

Supplier: Invitrogen

Description: The Mitochondria Isolation Kit for Cultured Cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples.

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