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Product Image-77392-724

Nanodisc MSP1D1 POPC

Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
Catalog Number: 77392-724
Supplier: 0000042190
Product Image-77392-468

PureCube DIBMA 10, Screening Kit, HEPES

Description: DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.
Catalog Number: 77392-468
Supplier: 0000042190
Product Image-77392-662

Human Recombinant MSP1D1 (from E. coli)

Description: Membrane scaffold protein, human origin.
Catalog Number: 77392-662
Supplier: 0000042190
Product Image-77392-660

Human Recombinant MSP1D1 dH5-His (from E. coli)

Description: Membrane scaffold protein, human origin.
Catalog Number: 77392-660
Supplier: 0000042190
Product Image-77392-472

PureCube DIBMA 12, Screening Kit, HEPES

Description: DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.
Catalog Number: 77392-472
Supplier: 0000042190
Product Image-77392-778

Mouse Recombinant MSP1E3D1-His (from E. coli)

Description: Membrane scaffold protein, mouse origin.
Catalog Number: 77392-778
Supplier: 0000042190
Product Image-77392-762

Nanodisc MSP1D1 dH5-His DMPC Biotinyl PE

Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
Catalog Number: 77392-762
Supplier: 0000042190
Product Image-77392-760

Nanodisc MSP1D1-His POPC Biotinyl PE

Description: This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
Catalog Number: 77392-760
Supplier: 0000042190
Image Unavailable-77392-856

SARS-CoV-2 Recombinant SARS CoV-2 Spike Protein Set in LMNG Detergent, DIBMA Glycerol, Nanodisc Complex (from HEK293 cells)

Description: The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.
Catalog Number: 77392-856
Supplier: 0000042190
Product Image-77392-864

SARS-CoV-2 Recombinant SARS-CoV-2 Spike Protein in SMALP 200 (from HEK293 cells)

Description: The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.
Catalog Number: 77392-864
Supplier: 0000042190
Product Image-77393-144

Anti-HighSpec Rho1D4 antibody

Description: This is an antibody specifically for targeting a Rho1D4-(TETSQVAPA)-tag on a protein.
Catalog Number: 77393-144
Supplier: CUBE BIOTECH
Image Unavailable-77392-542

AASTY 6-50

Description: AASTYs (Acrylic acid-co-styrenes) - like AASTY 6-50 - are highly-alternating copolymers, well-suited for the generation of native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 6-50 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-50 tend to be more aggressive, while heavier AASTYs, such as 11-45 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made using controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022
Catalog Number: 77392-542
Supplier: CUBE BIOTECH
Product Image-77393-366

PureCube Carboxy MagBeads

Description: PureCube amine carboxy agarose MagBeads are your best option to couple biomolecules that carry free-standing Amine (NH₂) groups.
Catalog Number: 77393-366
Supplier: CUBE BIOTECH
Product Image-77393-236

PureCube Maleimide Activated MagBeads

Description: PureCube Maleimide MagBeads are your best option to couple biomolecules that carry thiol (SH) groups.
Catalog Number: 77393-236
Supplier: CUBE BIOTECH
Product Image-77393-382

PureCube Epoxy Activated Agarose XL

Description: PureCube epoxy agarose XL resin is your best option to couple biomolecules that carry thiol (SH) groups.
Catalog Number: 77393-382
Supplier: CUBE BIOTECH
Product Image-77392-912

PureCube Ni-IDA MagBeads

Description: PureCube Ni-IDA MagBeads were developed by Cube Biotech for His-tag protein purification.
Catalog Number: 77392-912
Supplier: CUBE BIOTECH
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